IVF Embryo Production

A procedure that has now overtaken conventional embryo flushing worldwide in terms of embryos produced and is following the same trend at Embryo Plus.

Embryo Plus has proudly partnered with Vytelle, a renowned company with more than 14 laboratories worldwide at this stage.

Through Vytelle’s development of superior media (containing no foetal calf serum) and innovative new techniques and procedures, problems initially associated with IVFEP, such as large calves at birth or poor conceptions with frozen embryos, were eliminated. The Vytelle system also requires no hormones being used on the donor animals.

In Vitro Embryo Production (IVEP) in cattle consists of the following components:

Aspiration of oocytes:

The donors are aspirated (OPU) in order to harvest the oocytes from the ovaries. Ultrasound is used to visualize the ovaries and a guided needle is then used to puncture the follicles on the ovaries in order to retrieve the oocytes with suction.

Fertilization of oocytes in laboratory:

The oocytes are matured for 24 hours after the OPU’s, before they are fertilized in the laboratory.

Culturing of embryos in laboratory:

After fertilization, the embryos are cultured for 7 more days in incubators in the laboratory under very specific and strictly sterile conditions.

Transfer or freezing of embryos:

The embryos are graded and the grade 1 embryos may be transferred “fresh” into recipients, or frozen. Recipients are synchronized prior to transfer according to a hormone program supplied to the client by Embryo Plus. Any extra embryos will be frozen and stored for later use.

IVF-embryo production current standards using Vytelle’s system

Vytelle is an integrated technology platform built to accelerate genetic progress in cattle. Visit http://www.vytelle.com


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